Using LabVIEW, the Vision Development Module, and DAQ to Perform Deep-Tissue Cell Imaging

Solution by: Coleman Technologies, Inc.

Using the adaptive optics microscope with NI technology, researchers can obtain deeper tissue images with greater clarity than ever before.

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CTI is a NI Silver Alliance Partner with extensive expertise in scientific application development and serves numerous universities, foundations, medical device, and pharmaceutical companies. With several scientists with PhDs on staff, CTI is experienced in developing advanced imaging systems and custom image analysis software. The Challenge: Controlling a microscope that employs adaptive optics to reduce blurring in the imaging of deep-tissue biological samples by orders of magnitude relative to what is attainable with current state-of-the-art microscopy. The Solution: Using LabVIEW software, S Series data acquisition(DAQ) devices, a real-time system integration(RTSI) connection, and multiple third-party instruments to synchronously scan/acquire data, as well as the Vision Development Module to process data & send corrections to a spatial light modulator(SLM) in real time.

  • The two-photon microscope creates images by raster scanning the focus of a laser in a 2D plane within the sample
  • Adaptive optics is the technique used here to correct imaging aberrations
  • The technique can be adapted to correct for biological aberrations by initially taking many images to characterize the aberrations that each part of the light beam experiences as it travels through the sample
  • An SLM is then used to divide the light beam into sections and to steer the parts back into sharp focus
  • High-performance data analysis with native multicore support
  • Rapid prototyping and algorithm configuration for adaptation to new and changing requirements
  • Reliable operation
  • Elegant and customizable user interface
  • Easy integration with NI and third-party instruments
NI System Content
  • two NI PCI-6115 and one NI PCI-6110 S Series DAQ device
  • LabVIEW
  • Vision Development Module

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